验证数据展示
经过测试的应用
Positive WB detected in | U-87 MG cells |
Positive FC detected in | Transfected HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84018-4-RR targets FAP in WB, FC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | fibroblast activation protein, alpha |
别名 | FAPA, 170 kDa melanoma membrane-bound gelatinase, 241129B4, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP |
计算分子量 | 88 kDa |
观测分子量 | 80 kDa |
GenBank蛋白编号 | NM_004460.4 |
基因名称 | FAP |
Gene ID (NCBI) | 2191 |
RRID | AB_3671587 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q12884-1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).
实验方案
Product Specific Protocols | |
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WB protocol for FAP antibody 84018-4-RR | Download protocol |
FC protocol for FAP antibody 84018-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |