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Conjugate
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FAP Recombinant antibody

FAP Uni-rAbTM Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

241129B4

Cat no : 84018-4-RR

Print datasheet

Synonyms

FAPA, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form

验证数据展示

  • WB analysis of U-87 MG using 84018-4-RR

    WB analysis of U-87 MG using 84018-4-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of Transfected HEK-293T using 84018-4-RR

    FC experiment of Transfected HEK-293T using 84018-4-RR

    1x10^6 HEK-293T cells or Transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 84018-4-RR

    Affinity and Kinetic Characterization of 84018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

经过测试的应用

Positive WB detected inU-87 MG cells
Positive FC detected inTransfected HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy FAP Ready-To-Use IHC Kit. FAP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84018-4-RR is FAP Fusion Protein expressed in E. coli.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fibroblast activation protein, alpha
Synonyms FAPA, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form
Calculated Molecular Weight88 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberNM_004460.4
Gene Symbol FAP
Gene ID (NCBI) 2191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12884-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

实验方案

Product Specific Protocols
WB protocol for FAP antibody 84018-4-RRDownload protocol
FC protocol for FAP antibody 84018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols