验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells |
| Positive FC detected in | Transfected HEK-293T cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84018-4-RR targets FAPA in WB, FC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, FC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | fibroblast activation protein, alpha |
| Synonyms | FAP, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | NM_004460.4 |
| Gene Symbol | FAP |
| Gene ID (NCBI) | 2191 |
| RRID | AB_3671587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q12884-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAPA antibody 84018-4-RR | Download protocol |
| FC protocol for FAPA antibody 84018-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |