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Fas/CD95 Recombinant antibody

Fas/CD95 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240340E11

Cat no : 83400-5-RR

Print datasheet

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

验证数据展示

  • IF Staining of Raji using 83400-5-RR

    IF Staining of Raji using 83400-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of Raji using 83400-5-RR

    IF Staining of Raji using 83400-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83400-5-RR

    Affinity and Kinetic Characterization of 83400-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.

经过测试的应用

Positive IF/ICC detected inRaji cells, Jurkat cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83400-5-RR is Fas/CD95 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fas (TNF receptor superfamily, member 6)
Synonyms FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1
Calculated Molecular Weight38 kDa
GenBank Accession NumberNM_000043.6
Gene Symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25445-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

实验方案

Product Specific Protocols
IF protocol for Fas/CD95 antibody 83400-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols