CoraLite® Plus 488-conjugated Fas/CD95 Recombinant antibody
Fas/CD95 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240340E11
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 83400-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83400-5 is Fas/CD95 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | Fas (TNF receptor superfamily, member 6) |
| Synonyms | FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1 |
| Calculated Molecular Weight | 38 kDa |
| GenBank Accession Number | NM_000043.6 |
| Gene Symbol | Fas/CD95 |
| Gene ID (NCBI) | 355 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25445-1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Fas/CD95 antibody CL488-83400-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |