• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FASN Recombinant antibody, PBS Only

FASN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L9

Cat no : 81079-1-PBS

Print datasheet

Synonyms

3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase

验证数据展示

  • IF Staining of HeLa using 81079-1-RR (same clone as 81079-1-PBS)

    IF Staining of HeLa using 81079-1-RR (same clone as 81079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81079-1-PBS in a different storage buffer formulation.

  • WB analysis using 81079-1-RR (same clone as 81079-1-PBS)

    WB analysis using 81079-1-RR (same clone as 81079-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81079-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81079-1-RR

    Affinity and Kinetic Characterization of 81079-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81079-1-RR against Human FASN were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

81079-1-PBS targets FASN in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FASN fusion protein Ag0975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fatty acid synthase
Synonyms 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase
Calculated Molecular Weight 272 kDa
Observed Molecular Weight250-270 kDa
GenBank Accession NumberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.