• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13003-2-AP (same clone as 13003-2-PBS)

    WB analysis using 13003-2-AP (same clone as 13003-2-PBS)

    TNF alpha and IFN gamma treated HepG2 cells were subjected to SDS PAGE followed by western blot with 13003-2-AP (FAT10 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human tonsillitis using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human stomach cancer using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human stomach cancer using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human lymphoma using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human lymphoma using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human lung cancer using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human lung cancer using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • Featured Product
  • KD/KO验证

FAT10 Polyclonal antibody, PBS Only

FAT10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13003-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy UBD Ready-To-Use IHC Kit. UBD primary antibody included.

产品信息

13003-2-PBS targets FAT10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FAT10 fusion protein Ag3680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin D
别名
计算分子量 165 aa, 18 kDa
观测分子量20 kDa, 70kDa
GenBank蛋白编号BC012472
基因名称 FAT10
Gene ID (NCBI) 10537
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15205
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.