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FATP2 Monoclonal antibody

FATP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C10D7

Cat no : 68074-1-Ig

Print datasheet

Synonyms

SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase

验证数据展示

  • WB analysis using 68074-1-Ig

    WB analysis using 68074-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68074-1-Ig

    WB analysis of HepG2 using 68074-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 68074-1-Ig

    WB analysis of rat liver using 68074-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 68074-1-Ig

    IHC staining of human kidney using 68074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68074-1-Ig

    IHC staining of human kidney using 68074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68074-1-Ig

    IF Staining of HepG2 using 68074-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (68074-1-Ig, Clone: 2C10D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, rat liver tissue, LNCaP cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68074-1-Ig targets FATP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen FATP2 fusion protein Ag5390 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 27 (fatty acid transporter), member 2
Synonyms SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase
Calculated Molecular Weight 567 aa, 65 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC057770
Gene Symbol FATP2/SLC27A2
Gene ID (NCBI) 11001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14975
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

实验方案

Product Specific Protocols
WB protocol for FATP2 antibody 68074-1-IgDownload protocol
IHC protocol for FATP2 antibody 68074-1-IgDownload protocol
IF protocol for FATP2 antibody 68074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols