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验证数据展示

  • WB analysis using 68074-1-Ig (same clone as 68074-1-PBS)

    WB analysis using 68074-1-Ig (same clone as 68074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    WB analysis of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 68074-1-Ig (same clone as 68074-1-PBS)

    WB analysis of rat liver using 68074-1-Ig (same clone as 68074-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    IF Staining of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (68074-1-Ig, Clone: 2C10D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50832-1

    Cytometric bead array standard curve of MP50832-1

    Cytometric bead array standard curve of MP50832-1, SLC27A2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68074-1-PBS. Detection antibody: 68074-2-PBS. Standard:Ag5390. Range: 12.5-200 ng/mL.

FATP2 Monoclonal antibody, PBS Only (Capture)

FATP2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68074-1-PBS

产品说明书

CloneNo. 2C10D7

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68074-1-PBS targets FATP2 as part of a matched antibody pair:

MP50832-1: 68074-1-PBS capture and 68074-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 FATP2 fusion protein Ag5390 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 27 (fatty acid transporter), member 2
别名 SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase
计算分子量 567 aa, 65 kDa
观测分子量 70 kDa
GenBank蛋白编号BC057770
基因名称 FATP2/SLC27A2
Gene ID (NCBI) 11001
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14975
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.