验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84557-1-RR targets FATP5/SLC27A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | FATP5/SLC27A5 fusion protein Ag18276 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | solute carrier family 27 (fatty acid transporter), member 5 |
| Synonyms | FATP5, SLC27A5, Bile acyl-CoA synthetase, Bile acid-CoA ligase, BA-CoA ligase |
| Calculated Molecular Weight | 690 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC148807 |
| Gene Symbol | FATP5/SLC27A5 |
| Gene ID (NCBI) | 10998 |
| RRID | AB_3672066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9Y2P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FATP5/SLC27A5 antibody 84557-1-RR | Download protocol |
| IHC protocol for FATP5/SLC27A5 antibody 84557-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |