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Fibulin-1 Polyclonal antibody

Fibulin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20425-1-AP

Print datasheet

Synonyms

FBLN1, FBLN, PP213, fibulin 1, FIBL-1

验证数据展示

  • WB analysis of human placenta using 20425-1-AP

    WB analysis of human placenta using 20425-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20425-1-AP (Fibulin-1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20425-1-AP

    IF Staining of HeLa using 20425-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20425-1-AP (Fibulin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of human placenta using 20425-1-AP

    IP experiment of human placenta using 20425-1-AP

    IP result of anti-Fibulin-1 (IP:20425-1-AP, 4ug; Detection:20425-1-AP 1:20000) with human placenta tissue lysate 800 ug.

  • IHC staining of human stomach using 20425-1-AP

    IHC staining of human stomach using 20425-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20425-1-AP (Fibulin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20425-1-AP targets Fibulin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Fibulin-1 fusion protein Ag14249 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibulin 1
Synonyms FBLN1, FBLN, PP213, fibulin 1, FIBL-1
Calculated Molecular Weight 703 aa, 77 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC022497
Gene Symbol FBLN1
Gene ID (NCBI) 2192
RRIDAB_10859100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23142
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Fibulin-1 antibody 20425-1-APDownload protocol
IHC protocol for Fibulin-1 antibody 20425-1-APDownload protocol
IF protocol for Fibulin-1 antibody 20425-1-APDownload protocol
IP protocol for Fibulin-1 antibody 20425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics Clin Appl

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Authors - Yueyan Li
View Article
humanWB,IHC,IF

Comput Biol Med

Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma

Authors - Guokun Zhang
View Article