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验证数据展示

  • WB analysis of HeLa using 83509-6-RR (same clone as 83509-6-PBS)

    WB analysis of HeLa using 83509-6-RR (same clone as 83509-6-PBS)

    WB result of FBXO38 antibody (83509-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO38 transfected HeLa cells. This data was developed using the same antibody clone with 83509-6-PBS in a different storage buffer formulation.

  • WB analysis using 83509-6-RR (same clone as 83509-6-PBS)

    WB analysis using 83509-6-RR (same clone as 83509-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83509-6-RR (FBXO38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83509-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83509-6-RR (same clone as 83509-6-PBS)

    FC experiment of HeLa using 83509-6-RR (same clone as 83509-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FBXO38 Recombinant antibody (83509-6-RR, Clone:240513C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83509-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83509-6-RR

    Affinity and Kinetic Characterization of 83509-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83509-6-RR against Human FBXO38 were performed. The affinity constant is 0.723 nM.

  • Featured Product
  • KD/KO验证

FBXO38 Recombinant antibody, PBS Only

FBXO38 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83509-6-PBS

产品说明书

CloneNo. 240513C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83509-6-PBS targets FBXO38 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FBXO38 fusion protein Ag35444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F-box protein 38
别名 SP329, MOKA, Fbx38, F box protein 38, F box only protein 38
计算分子量134kDa，1188aa
观测分子量133 kDa
GenBank蛋白编号XM_005268513.1
基因名称 FBXO38
Gene ID (NCBI) 81545
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6PIJ6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXO38, also known as HMN2D, MOKA, and SP329, is a protein that plays a significant role in the regulation of immune responses, particularly in the context of cancer and autoimmune diseases. FBXO38 has been identified as an E3 ubiquitin ligase that mediates the degradation of PD-1 (Programmed cell death protein 1), a key immune checkpoint protein expressed on the surface of T cells. The internalization, ubiquitination, and proteasome degradation of PD-1 are facilitated by FBXO38, which links PD-1 to Lys48, leading to its poly-ubiquitination and subsequent degradation (PMID: 30487606).