• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HEK-293 using CL488-67697

    IF Staining of HEK-293 using CL488-67697

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 FBXO6 antibody (CL488-67697, Clone: 3B7F9 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-67697

    FC experiment of Jurkat using CL488-67697

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FBXO6 (CL488-67697, Clone:3B7F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FBXO6 Monoclonal antibody

FBXO6 Monoclonal Antibody for IF/ICC
Cat No. CL488-67697

产品说明书

CloneNo. 3B7F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC

别名

F-box protein that recognizes sugar chains 2, F-box only protein 6, FBG2, F box protein 6, F box only protein 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67697-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67697 targets FBXO6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 FBXO6 fusion protein Ag30512 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 F-box protein 6
别名 F-box protein that recognizes sugar chains 2, F-box only protein 6, FBG2, F box protein 6, F box only protein 6
计算分子量 293 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC020880
基因名称 FBXO6
Gene ID (NCBI) 26270
RRIDAB_2923782
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRD1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FBXO6 antibody CL488-67697Download protocol
Standard Protocols
Click here to view our Standard Protocols