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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 30356-1-AP (same clone as 30356-1-PBS)

    IF Staining of mouse spleen using 30356-1-AP (same clone as 30356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FCGR3A/CD16a antibody (30356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

FCGR3A/CD16a Polyclonal antibody, PBS Only

FCGR3A/CD16a Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 30356-1-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Synonyms

CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

30356-1-PBS targets FCGR3A/CD16a in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen FCGR3A/CD16a fusion protein Ag1281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen
Calculated Molecular Weight 29 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC036723
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.