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FDX1 Recombinant antibody

FDX1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230196E9

Cat No : 82957-2-RR

Print datasheet

Synonyms

Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, Hepatoredoxin, LOH11CR1D

验证数据展示

  • WB analysis using 82957-2-RR

    WB analysis using 82957-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82957-2-RR

    IF Staining of HepG2 using 82957-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-2-RR, Clone: 230196E9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, PC-3 cells, U-87 MG cells, U-251 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82957-2-RR targets FDX1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen FDX1 fusion protein Ag3301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ferredoxin 1
Synonyms Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, Hepatoredoxin, LOH11CR1D
Calculated Molecular Weight 184 aa, 19 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC017063
Gene Symbol FDX1
Gene ID (NCBI) 2230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

实验方案

Product Specific Protocols
WB protocol for FDX1 antibody 82957-2-RRDownload protocol
IF protocol for FDX1 antibody 82957-2-RRDownload protocol
FC protocol for FDX1 antibody 82957-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols