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验证数据展示

  • IF Staining of PC-3 using 83810-1-RR (same clone as 83810-1-PBS)

    IF Staining of PC-3 using 83810-1-RR (same clone as 83810-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83810-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83810-1-RR (same clone as 83810-1-PBS)

    FC experiment of A431 using 83810-1-RR (same clone as 83810-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FEM1B Recombinant antibody (83810-1-RR, Clone:240607D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83810-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 83810-1-RR (same clone as 83810-1-PBS)

    IF Staining of PC-3 using 83810-1-RR (same clone as 83810-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83810-1-PBS in a different storage buffer formulation.

FEM1B Recombinant antibody, PBS Only

FEM1B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83810-1-PBS

产品说明书

CloneNo. 240607D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83810-1-PBS targets FEM1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fem-1 homolog b (C. elegans)
别名 FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha
计算分子量 70 kDa
GenBank蛋白编号NM_015322
基因名称 FEM1B
Gene ID (NCBI) 10116
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UK73
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.