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FEM1B Recombinant antibody

FEM1B Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240607D8

Cat No : 83810-1-RR

Print datasheet

Synonyms

FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha

验证数据展示

  • IF Staining of PC-3 using 83810-1-RR

    IF Staining of PC-3 using 83810-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 83810-1-RR

    FC experiment of A431 using 83810-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FEM1B Recombinant antibody (83810-1-RR, Clone:240607D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of PC-3 using 83810-1-RR

    IF Staining of PC-3 using 83810-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83810-1-RR targets FEM1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fem-1 homolog b (C. elegans)
Synonyms FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha
Calculated Molecular Weight 70 kDa
GenBank Accession NumberNM_015322
Gene Symbol FEM1B
Gene ID (NCBI) 10116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UK73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

实验方案

Product Specific Protocols
IF protocol for FEM1B antibody 83810-1-RRDownload protocol
FC protocol for FEM1B antibody 83810-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols