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验证数据展示

  • WB analysis of HepG2 using 83536-1-RR

    WB analysis of HepG2 using 83536-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83536-1-RR

    FC experiment of U2OS using 83536-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83536-1-RR

    Affinity and Kinetic Characterization of 83536-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

FEM1C Recombinant antibody

FEM1C Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83536-1-RR

Print datasheet

CloneNo. 240126G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83536-1-RR targets FEM1C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FEM1C fusion protein Ag5099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fem-1 homolog c (C. elegans)
Synonyms FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
RRIDAB_3671160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96JP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

实验方案

Product Specific Protocols
WB protocol for FEM1C antibody 83536-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols