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FEM1C Recombinant antibody, PBS Only

FEM1C Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240126G5

Cat no : 83536-1-PBS

Print datasheet

Synonyms

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

验证数据展示

  • WB analysis of HepG2 using 83536-1-RR (same clone as 83536-1-PBS)

    WB analysis of HepG2 using 83536-1-RR (same clone as 83536-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83536-1-RR (same clone as 83536-1-PBS)

    FC experiment of U2OS using 83536-1-RR (same clone as 83536-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83536-1-RR

    Affinity and Kinetic Characterization of 83536-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

产品信息

The immunogen of 83536-1-PBS is FEM1C Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FEM1C fusion protein Ag5099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fem-1 homolog c (C. elegans)
Synonyms FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96JP0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.