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验证数据展示

  • FC experiment of Jurkat using CL488-25287

    FC experiment of Jurkat using CL488-25287

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FER (CL488-25287) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated FER Polyclonal antibody

FER Polyclonal Antibody for FC (Intra)
Cat No. CL488-25287

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

Proto-oncogene c-Fer, p94-Fer, p94 Fer, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Feline encephalitis virus-related kinase FER

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FER Ready-To-Use IHC Kit. FER primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25287-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25287 targets FER in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 FER fusion protein Ag18173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fer (fps/fes related) tyrosine kinase
别名 Proto-oncogene c-Fer, p94-Fer, p94 Fer, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Feline encephalitis virus-related kinase FER
计算分子量 822 aa, 95 kDa
观测分子量 95 kDa
GenBank蛋白编号BC017060
基因名称 FER
Gene ID (NCBI) 2241
RRIDAB_2919202
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16591
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FER antibody CL488-25287Download protocol
Standard Protocols
Click here to view our Standard Protocols