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- KD/KO Validated
Kindlin 2 Polyclonal antibody
Kindlin 2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FERMT2/Kindlin 2 Ready-To-Use IHC Kit. FERMT2/Kindlin 2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11453-1-AP targets Kindlin 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, hamster |
| Immunogen | Kindlin 2 fusion protein Ag2013 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fermitin family homolog 2 (Drosophila) |
| Synonyms | Fermitin family homolog 2, FERMT2, KIND2, Kindlin 2, mig 2, MIG2, PLEKHC1, UNC112, UNC112B |
| Calculated Molecular Weight | 680 aa, 78 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC017327 |
| Gene Symbol | Kindlin 2 |
| Gene ID (NCBI) | 10979 |
| RRID | AB_2262660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AC1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IHC protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IP protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. | ||
Circ Res Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
| ||
Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis | ||
J Am Soc Nephrol Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.
| ||
J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
| ||
J Cell Sci Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration
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