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FEZ2 Recombinant antibody

FEZ2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240248B10

Cat no : 83407-7-RR

Print datasheet

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

验证数据展示

  • WB analysis using 83407-7-RR

    WB analysis using 83407-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83407-7-RR

    IHC staining of human ovary cancer using 83407-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 83407-7-RR

    FC experiment of A431 using 83407-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83407-7-RR

    Affinity and Kinetic Characterization of 83407-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, A375 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83407-7-RR targets FEZ2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FEZ2 fusion protein Ag15370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fasciculation and elongation protein zeta 2 (zygin II)
Synonyms Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2
Calculated Molecular Weight 353 aa, 40 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC110386
Gene Symbol FEZ2
Gene ID (NCBI) 9637
RRIDAB_3671055
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UHY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.

实验方案

Product Specific Protocols
WB protocol for FEZ2 antibody 83407-7-RRDownload protocol
IHC protocol for FEZ2 antibody 83407-7-RRDownload protocol
FC protocol for FEZ2 antibody 83407-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols