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FFAR2 Recombinant antibody, PBS Only

FFAR2 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241696B4

Cat no : 84544-1-PBS

Print datasheet

Synonyms

GPCR43, G protein coupled receptor 43, free fatty acid receptor 2, FFA2R, FFA2

验证数据展示

  • WB analysis using 84544-1-RR (same clone as 84544-1-PBS)

    WB analysis using 84544-1-RR (same clone as 84544-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84544-1-RR (FFAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84544-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84544-1-RR (same clone as 84544-1-PBS)

    IF Staining of mouse brain using 84544-1-RR (same clone as 84544-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FFAR2 antibody (84544-1-RR, Clone: 241696B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84544-1-PBS in a different storage buffer formulation.

产品信息

84544-1-PBS targets FFAR2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name free fatty acid receptor 2
Synonyms GPCR43, G protein coupled receptor 43, free fatty acid receptor 2, FFA2R, FFA2
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC096198
Gene Symbol FFAR2
Gene ID (NCBI) 2867
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15552
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).