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验证数据展示

  • WB analysis of mouse brain using 60152-1-Ig

    WB analysis of mouse brain using 60152-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 60152-1-Ig

    FC experiment of HeLa using 60152-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug FGF12 Monoclonal antibody (60152-1-Ig, Clone:2F11A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FGF12 Monoclonal antibody

FGF12 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 60152-1-Ig

产品说明书

CloneNo. 2F11A10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA, FC (Intra)

别名

FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60152-1-Ig targets FGF12 in WB, ELISA, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 human
免疫原 FGF12 fusion protein Ag5046 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor 12
别名 FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor
计算分子量 181 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC022524
基因名称 FGF12
Gene ID (NCBI) 2257
RRIDAB_10644325
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61328
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

实验方案

Product Specific Protocols
WB protocol for FGF12 antibody 60152-1-IgDownload protocol
FC protocol for FGF12 antibody 60152-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols