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验证数据展示

  • WB analysis of HT-29 using 84536-3-RR

    WB analysis of HT-29 using 84536-3-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84536-3-RR

    Affinity and Kinetic Characterization of 84536-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

FGF19 Recombinant antibody

FGF19 Recombinant Antibody for WB, ELISA
Cat No. 84536-3-RR

Print datasheet

CloneNo. 241740E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84536-3-RR targets FGF19 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FGF19 fusion protein HZ-1330 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fibroblast growth factor 19
Synonyms UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC017664
Gene Symbol FGF19
Gene ID (NCBI) 9965
RRIDAB_3672043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95750
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .

实验方案

Product Specific Protocols
WB protocol for FGF19 antibody 84536-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols