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验证数据展示

  • WB analysis of HT-29 using 84536-3-RR (same clone as 84536-3-PBS)

    WB analysis of HT-29 using 84536-3-RR (same clone as 84536-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84536-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01398-1

    Sandwich ELISA standard curve of MP01398-1

    Sandwich ELISA standard curve of MP01398-1, Human FGF19 Recombinant Matched Antibody Pair - PBS only. 84536-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1330. 84536-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-1000 pg/mL.

  • Sample test of MP01398-1

    Sample test of MP01398-1

    HT-29 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human FGF19, and measured 32.7 ng/mL.

  • Sample test of MP01398-1

    Sample test of MP01398-1

    Serum of six individual healthy human donors was measured. The FGF19 concentration of detected samples was determined to be 199.4 pg/mL with a range of 136.6-250.1 pg/mL.

  • Cytometric bead array standard curve of MP01398-1

    Cytometric bead array standard curve of MP01398-1

    Cytometric bead array standard curve of MP01398-1, FGF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84536-3-PBS. Detection antibody: 84536-2-PBS. Standard: HZ-1330. Range: 0.078-20 ng/mL.

  • Affinity and Kinetic Characterization of 84536-3-RR

    Affinity and Kinetic Characterization of 84536-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

FGF19 Recombinant antibody, PBS Only (Capture)

FGF19 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84536-3-PBS

产品说明书

CloneNo. 241740E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84536-3-PBS targets FGF19 as part of a matched antibody pair:

MP01398-1: 84536-3-PBS capture and 84536-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 FGF19 fusion protein HZ-1330 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibroblast growth factor 19
别名 UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4
计算分子量 216 aa, 24 kDa
观测分子量24 kDa
GenBank蛋白编号BC017664
基因名称 FGF19
Gene ID (NCBI) 9965
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95750
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .