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验证数据展示

  • WB analysis using 84205-4-RR (same clone as 84205-4-PBS)

    WB analysis using 84205-4-RR (same clone as 84205-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84205-4-RR (FGFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84205-4-RR (same clone as 84205-4-PBS)

    IHC staining of mouse liver using 84205-4-RR (same clone as 84205-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84205-4-RR (FGFR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01128-1

    Cytometric bead array standard curve of MP01128-1

    Cytometric bead array standard curve of MP01128-1, FGFR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84205-3-PBS. Detection antibody: 84205-4-PBS. Standard: Eg1265. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01128-3

    Cytometric bead array standard curve of MP01128-3

    Cytometric bead array standard curve of MP01128-3, FGFR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84205-2-PBS. Detection antibody: 84205-4-PBS. Standard: Eg1265. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84205-4-RR

    Affinity and Kinetic Characterization of 84205-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84205-4-RR against Human FGFR2 were performed. The affinity constant is 18.8 nM.

FGFR2 Recombinant antibody, PBS Only (Detector)

FGFR2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 84205-4-PBS

产品说明书

CloneNo. 241383G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

CFD1, CEK3, CD332, BFR 1, BEK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84205-4-PBS targets FGFR2 as part of a matched antibody pair:

MP01128-1: 84205-3-PBS capture and 84205-4-PBS detection (validated in Cytometric bead array)

MP01128-3: 84205-2-PBS capture and 84205-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibroblast growth factor receptor 2
别名 CFD1, CEK3, CD332, BFR 1, BEK
计算分子量92kd
观测分子量85 kDa
GenBank蛋白编号NM_000141.4
基因名称 FGFR2
Gene ID (NCBI) 2263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21802
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.