FGFR2 Recombinant antibody

FGFR2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241383G9

Cat no : 84205-4-RR

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Synonyms

CFD1, CEK3, CD332, BFR 1, BEK



经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84205-4-RR targets FGFR2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fibroblast growth factor receptor 2
Synonyms CFD1, CEK3, CD332, BFR 1, BEK
Calculated Molecular Weight92kd
Observed Molecular Weight85 kDa
GenBank Accession NumberNM_000141.4
Gene Symbol FGFR2
Gene ID (NCBI) 2263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

实验方案

Product Specific Protocols
WB protocol for FGFR2 antibody 84205-4-RRDownload protocol
IHC protocol for FGFR2 antibody 84205-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols