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验证数据展示

  • WB analysis of L02 using 66158-1-Ig

    WB analysis of L02 using 66158-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 66158-1-Ig

    IF Staining of L02 using 66158-1-Ig

    Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • WB analysis of human peripheral blood leukocyte using 66158-1-Ig

    WB analysis of human peripheral blood leukocyte using 66158-1-Ig

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human peripheral blood platelets using 66158-1-Ig

    WB analysis of human peripheral blood platelets using 66158-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66158-1-Ig

    IHC staining of human liver cancer using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66158-1-Ig

    IHC staining of human liver cancer using 66158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Fibrinogen Gamma Chain Monoclonal antibody

Fibrinogen Gamma Chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66158-1-Ig

Print datasheet

CloneNo. 2B2B8
Publications(2)

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FGG, PRO2061, 2B2B8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman peripheral blood leukocyte, L02 cells, human peripheral blood platelets
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66158-1-Ig targets Fibrinogen Gamma Chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Fibrinogen Gamma Chain fusion protein Ag8817 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name fibrinogen gamma chain
Synonyms FGG, PRO2061, 2B2B8
Calculated Molecular Weight453aa,52 kDa; 437aa,50 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007044
Gene Symbol Fibrinogen Gamma Chain
Gene ID (NCBI) 2266
RRIDAB_2881554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02679
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

实验方案

Product Specific Protocols
WB protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IHC protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IF protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer.

Authors - H H Peng
View Article

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li
View Article