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验证数据展示

  • IF Staining of HepG2 using CL594-67391

    IF Staining of HepG2 using CL594-67391

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 FGL1 antibody (CL594-67391, Clone: 2B11C6 ) at dilution of 1:200.

CoraLite®594-conjugated FGL1 Monoclonal antibody

FGL1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67391

Print datasheet

CloneNo. 2B11C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF/ICC

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67391-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67391 targets FGL1 in IF/ICC applications and shows reactivity with Human, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, pig
Immunogen FGL1 fusion protein Ag8581 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibrinogen-like 1
Synonyms FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1
Calculated Molecular Weight 312 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC007047
Gene Symbol FGL1
Gene ID (NCBI) 2267
RRIDAB_3673517
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ08830
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrinogen-like 1 (FGL1), also termed hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or hepassocin, is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

实验方案

Product Specific Protocols
IF protocol for CL594 FGL1 antibody CL594-67391Download protocol
Standard Protocols
Click here to view our Standard Protocols