验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, Jurkat cells |
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67152-1-Ig targets FGL2 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | FGL2 fusion protein Ag28619 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fibrinogen-like 2 |
| 别名 | FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC017813 |
| 基因名称 | FGL2 |
| Gene ID (NCBI) | 10875 |
| RRID | AB_2882449 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14314 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGL2 antibody 67152-1-Ig | Download protocol |
| IF protocol for FGL2 antibody 67152-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma | ||
Cell Rep FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation |
