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FH Monoclonal antibody

FH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C8

Cat no : 68162-1-Ig

Print datasheet

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

验证数据展示

  • WB analysis using 68162-1-Ig

    WB analysis using 68162-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 68162-1-Ig

    WB analysis of HepG2 using 68162-1-Ig

    WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68162-1-Ig targets FH in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen FH fusion protein Ag30254 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fumarate hydratase
Synonyms HsFH, Fumarase, EC:4.2.1.2, 1A9C8
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
WB protocol for FH antibody 68162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols