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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FH Monoclonal antibody

FH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9C8

Cat no : CL488-68162

Print datasheet

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

验证数据展示

  • IF Staining of HeLa using CL488-68162

    IF Staining of HeLa using CL488-68162

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 FH antibody (CL488-68162, Clone: 1A9C8 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68162-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68162 targets FH in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen FH fusion protein Ag30254 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fumarate hydratase
Synonyms FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FH antibody CL488-68162Download protocol
Standard Protocols
Click here to view our Standard Protocols