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  • Featured Product
  • KD/KO Validated

FH Recombinant antibody, PBS Only

FH Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K16

Cat no : 82508-1-PBS

Print datasheet

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 3K16

验证数据展示

  • WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.

产品信息

82508-1-PBS targets FH in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FH fusion protein Ag1934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fumarate hydratase
Synonyms HsFH, Fumarase, EC:4.2.1.2, 3K16
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.