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  • Featured Product
  • KD/KO Validated

FH Recombinant antibody

FH Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3K16

Cat no : 82508-1-RR

Print datasheet

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 3K16

验证数据展示

  • WB analysis using 82508-1-RR

    WB analysis using 82508-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82508-1-RR

    WB analysis of HepG2 using 82508-1-RR

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

  • IP experiment of mouse liver using 82508-1-RR

    IP experiment of mouse liver using 82508-1-RR

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug.

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82508-1-RR targets FH in WB, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen FH fusion protein Ag1934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fumarate hydratase
Synonyms HsFH, Fumarase, EC:4.2.1.2, 3K16
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

实验方案

Product Specific Protocols
WB protocol for FH antibody 82508-1-RRDownload protocol
IP protocol for FH antibody 82508-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols