FHIT Recombinant antibody
FHIT Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240564D9
验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HL-60 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83710-1-RR targets FHIT in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | FHIT fusion protein Ag4181 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | fragile histidine triad gene |
| Synonyms | AP3Aase, AP3A hydrolase, Adenylylsulfate-ammonia adenylyltransferase, Adenylylsulfatase, Adenosine 5'-monophosphoramidase FHIT |
| Calculated Molecular Weight | 147 aa, 17 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC032336 |
| Gene Symbol | FHIT |
| Gene ID (NCBI) | 2272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49789 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FHIT antibody 83710-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |