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FIP1L1 Polyclonal antibody, PBS Only

FIP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12276-1-PBS

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Synonyms

验证数据展示

  • WB analysis of K-562 using 12276-1-AP (same clone as 12276-1-PBS)

    WB analysis of K-562 using 12276-1-AP (same clone as 12276-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

    IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FIP1L1 antibody included
  • IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FIP1L1 antibody included
Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

产品信息

12276-1-PBS targets FIP1L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FIP1L1 fusion protein Ag2921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FIP1 like 1 (S. cerevisiae)
Synonyms
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
RRIDAB_2231572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UN15
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).