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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of K-562 using 12276-1-AP (same clone as 12276-1-PBS)

    WB analysis of K-562 using 12276-1-AP (same clone as 12276-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

    IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

  • WB analysis using 12276-1-AP (same clone as 12276-1-PBS)

    WB analysis using 12276-1-AP (same clone as 12276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FIP1L1 antibody included
  • IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FIP1L1 antibody included

FIP1L1 Polyclonal antibody, PBS Only

FIP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12276-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

产品信息

12276-1-PBS targets FIP1L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FIP1L1 fusion protein Ag2921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FIP1 like 1 (S. cerevisiae)
别名 FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE
计算分子量 594 aa, 66 kDa
观测分子量 67 kDa
GenBank蛋白编号BC017724
基因名称 FIP1L1
Gene ID (NCBI) 81608
RRIDAB_2231572
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UN15
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).