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FIP1L1 Recombinant antibody, PBS Only

FIP1L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240658C4

Cat no : 83863-2-PBS

Print datasheet

Synonyms

RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1, 240658C4

验证数据展示

  • WB analysis using 83863-2-RR (same clone as 83863-2-PBS)

    WB analysis using 83863-2-RR (same clone as 83863-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83863-2-RR (same clone as 83863-2-PBS)

    FC experiment of A549 using 83863-2-RR (same clone as 83863-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 83863-2-RR (same clone as 83863-2-PBS)

    FC experiment of U-2 OS using 83863-2-RR (same clone as 83863-2-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83863-2-RR

    Affinity and Kinetic Characterization of 83863-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

产品信息

The immunogen of 83863-2-PBS is FIP1L1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen FIP1L1 fusion protein Ag2921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name FIP1 like 1 (S. cerevisiae)
Synonyms RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1, 240658C4
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 67-80 kDa
GenBank Accession NumberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6UN15
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).