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验证数据展示

  • IHC staining of human ovary cancer using 83863-3-RR (same clone as 83863-3-PBS)

    IHC staining of human ovary cancer using 83863-3-RR (same clone as 83863-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83863-3-RR (FIP1L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83863-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FIP1L1 antibody included
  • IF Staining of MCF-7 using 83863-3-RR (same clone as 83863-3-PBS)

    IF Staining of MCF-7 using 83863-3-RR (same clone as 83863-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (83863-3-RR, Clone: 240658A8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83863-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83863-3-RR (same clone as 83863-3-PBS)

    FC experiment of A549 using 83863-3-RR (same clone as 83863-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-3-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 83863-3-RR (same clone as 83863-3-PBS)

    FC experiment of U-2 OS using 83863-3-RR (same clone as 83863-3-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83863-3-RR

    Affinity and Kinetic Characterization of 83863-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83863-3-RR against Human FIP1L1 were performed. The affinity constant is 1.63 nM.

FIP1L1 Recombinant antibody, PBS Only

FIP1L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83863-3-PBS

产品说明书

CloneNo. 240658A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

产品信息

83863-3-PBS targets FIP1L1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FIP1L1 fusion protein Ag2921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FIP1 like 1 (S. cerevisiae)
别名 240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE
计算分子量 594 aa, 66 kDa
GenBank蛋白编号BC017724
基因名称 FIP1L1
Gene ID (NCBI) 81608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6UN15
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).