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FITC Plus Mouse IgG2a Isotype Control (C1.18.4)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

C1.18.4

Cat no : FITC-65208

Print datasheet

Synonyms

FITC Mouse IgG2a Isotype Control, IgG2a, IgG2a Isotype Control

验证数据展示

  • FC experiment of human PBMCs using FITC-65208

    FC experiment of human PBMCs using FITC-65208

    1X10^6 human PBMCs were surface stained with 0.2 ug FITC Plus Anti-Mouse IgG2a Isotype Control (FITC-65208, Clone: C1.18.4) (red), or FITC Plus Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs, mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name IgG2a Isotype Control
Synonyms FITC Mouse IgG2a Isotype Control, IgG2a, IgG2a Isotype Control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883822
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for FITC Plus IgG2a Isotype Control antibody FITC-65208Download protocol
Standard Protocols
Click here to view our Standard Protocols