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ChromoTek FITC Plus-conjugated TIM3 VHH

FITC-tmt targets TIM3 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

FITC Plus Fluorescent dye

Cat No : FITC-tmt

Synonyms

TIM3-Nanobody, TIM3-single domain antibody (sdAb), CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

Validation Data Gallery

  • 1X10^6 HEK TIM3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg FITC Plus-conjugated TIM3 VHH (FITC-tmt). Cells were not fixed.

    1X10^6 HEK TIM3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg FITC Plus-conjugated TIM3 VHH (FITC-tmt). Cells were not fixed.

  • Live HeLa cells transfected with human TIM-3 were immunostained with FITC Plus-conjugated TIM3 VHH (FITC-tmt,1:1000, green). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with FITC-tmt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human TIM-3 were immunostained with FITC Plus-conjugated TIM3 VHH (FITC-tmt,1:1000, green). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with 20x objective and post-processed.  Note: Immunostaining with FITC-tmt can also be performed after formaldehyde fixation of the cells.

  • Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

    Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 1ug FITC Plus conjugated TIM-3 VHH (FITC-tmt) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

Product Information

FITC-tmt targets TIM3 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateFITC Plus Fluorescent dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.824 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
RRIDAB_3101923
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C; Shipped at ambient temperature.
Background TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

Documentation

SDS
SDS-Immuno-Oncology VHHs