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  • Featured Product
  • KD/KO Validated

FKBP10/FKBP65 Monoclonal antibody

FKBP10/FKBP65 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D9

Cat no : 68085-1-Ig

Print datasheet

Synonyms

FKBP10, FKBP65, FKBP 10, FK506-binding protein 10, FK506 binding protein 10

验证数据展示

  • WB analysis using 68085-1-Ig

    WB analysis using 68085-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68085-1-Ig (FKBP10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HeLa using 68085-1-Ig

    WB analysis of HeLa using 68085-1-Ig

    WB result of FKBP10/FKBP65 antibody (68085-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, MCF-7 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68085-1-Ig targets FKBP10/FKBP65 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FKBP10/FKBP65 fusion protein Ag31628 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 10, 65 kDa
Synonyms FKBP10, FKBP65, FKBP 10, FK506-binding protein 10, FK506 binding protein 10
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight64-70 kDa
GenBank Accession NumberBC016467
Gene Symbol FKBP10
Gene ID (NCBI) 60681
RRIDAB_2918822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96AY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

实验方案

Product Specific Protocols
WB protocol for FKBP10/FKBP65 antibody 68085-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols