验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat testis tissue, mouse testis tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67874-1-Ig targets FKBP5 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, rat, mouse |
| 文献引用反应性 | human |
| 免疫原 | FKBP5 fusion protein Ag5514 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 5 |
| 别名 | 51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase |
| 计算分子量 | 457 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC042605 |
| 基因名称 | FKBP5 |
| Gene ID (NCBI) | 2289 |
| RRID | AB_2918632 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13451 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP5 antibody 67874-1-Ig | Download protocol |
| IHC protocol for FKBP5 antibody 67874-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
