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验证数据展示

  • FC experiment of K-562 using CL488-67874

    FC experiment of K-562 using CL488-67874

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FKBP5 (CL488-67874, Clone:3B11F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67874

    IF Staining of HeLa using CL488-67874

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FKBP5 antibody (CL488-67874, Clone: 3B11F3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated FKBP5 Monoclonal antibody

FKBP5 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67874

产品说明书

CloneNo. 3B11F3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Androgen-regulated protein 6, AIG6, 54 kDa progesterone receptor-associated immunophilin, 51 kDa FK506-binding protein, 3B11F3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67874-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67874 targets FKBP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 FKBP5 fusion protein Ag5514 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 5
别名 Androgen-regulated protein 6, AIG6, 54 kDa progesterone receptor-associated immunophilin, 51 kDa FK506-binding protein, 3B11F3
计算分子量 457 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC042605
基因名称 FKBP5
Gene ID (NCBI) 2289
RRIDAB_2923812
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13451
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FKBP5 antibody CL488-67874Download protocol
Standard Protocols
Click here to view our Standard Protocols