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验证数据展示

  • WB analysis using 19163-1-AP

    WB analysis using 19163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19163-1-AP (FKBP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 19163-1-AP

    IF Staining of MCF-7 using 19163-1-AP

    Immunofluorescent analysis of MCF-7 cells, using FKBP6 antibody 19163-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

FKBP6 Polyclonal antibody

FKBP6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19163-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

36 kDa FK506 binding protein, 36 kDa FKBP, FK506 binding protein 6, FK506 binding protein 6, 36kDa, FKBP 36, FKBP 6, FKBP36, FKBP6, Immunophilin FKBP36, PPIase, PPIase FKBP6, Rotamase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, human testis tissue, mouse testis tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19163-1-AP targets FKBP6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FKBP6 fusion protein Ag4642 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 6, 36kDa
别名 36 kDa FK506 binding protein, 36 kDa FKBP, FK506 binding protein 6, FK506 binding protein 6, 36kDa, FKBP 36, FKBP 6, FKBP36, FKBP6, Immunophilin FKBP36, PPIase, PPIase FKBP6, Rotamase
计算分子量 327 aa, 37 kDa
观测分子量 36 kDa
GenBank蛋白编号BC036817
基因名称 FKBP6
Gene ID (NCBI) 8468
RRIDAB_10603363
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75344
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inactive peptidyl-prolyl cis-trans isomerase FKBP6 (FKBP6), also known as 36 kDa FK506-binding protein (36 kDa FKBP; FKBP-36), is a member of a gene family that contains a prolyl isomerase/FK506 binding domain and tetratricopeptide protein-protein interaction domains(PMID: 9782077). It has been linked to the secondary piRNA biogenesis in fetal male mouse germ cells ( PMID: 22902560).

实验方案

Product Specific Protocols
WB protocol for FKBP6 antibody 19163-1-APDownload protocol
IF protocol for FKBP6 antibody 19163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols