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  • Featured Product
  • KD/KO Validated

FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1C1G12

Cat no : 66690-1-Ig

Print datasheet

Synonyms

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

验证数据展示

  • IHC staining of human hypothalamus using 66690-1-Ig

    IHC staining of human hypothalamus using 66690-1-Ig

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 66690-1-Ig

    WB analysis of Jurkat using 66690-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66690-1-Ig

    WB analysis using 66690-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 66690-1-Ig

    IF Staining of A431 using 66690-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66690-1-Ig targets FKBP8 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen FKBP8 fusion protein Ag1649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 8, 38kDa
Synonyms FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_2882044
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FKBP8 antibody 66690-1-IgDownload protocol
IHC protocol for FKBP8 antibody 66690-1-IgDownload protocol
IF protocol for FKBP8 antibody 66690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

bioRxiv

Tau phosphorylation suppresses oxidative stress-induced mitophagy via FKBP8 receptor modulation

Authors - Michael O Isei
  • KD Validated
View Article
humanCoIP

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
View Article