FKBPL Monoclonal antibody

FKBPL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H10B10

Cat no : 66389-1-Ig

Print datasheet

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1



经过测试的应用

Positive WB detected inMCF-10A cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, Calu-3 cells, Caco-2 cells, HepG2 cells, C6 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66389-1-Ig targets FKBPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FKBPL fusion protein Ag0112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein like
Synonyms WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2881765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UIM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

实验方案

Product Specific Protocols
WB protocol for FKBPL antibody 66389-1-IgDownload protocol
IHC protocol for FKBPL antibody 66389-1-IgDownload protocol
IF protocol for FKBPL antibody 66389-1-IgDownload protocol
FC protocol for FKBPL antibody 66389-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia

Authors - Sahar Masoumeh Ghorbanpour