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FKBPL Monoclonal antibody, PBS Only

FKBPL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10B10

Cat no : 66389-1-PBS

Print datasheet

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

验证数据展示

  • WB analysis using 66389-1-Ig (same clone as 66389-1-PBS)

    WB analysis using 66389-1-Ig (same clone as 66389-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

    IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

    IHC staining of human breast cancer using 66389-1-Ig (same clone as 66389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66389-1-Ig (same clone as 66389-1-PBS)

    IF Staining of MCF-7 using 66389-1-Ig (same clone as 66389-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66389-1-Ig (same clone as 66389-1-PBS)

    FC experiment of HeLa using 66389-1-Ig (same clone as 66389-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66389-1-PBS in a different storage buffer formulation.

产品信息

66389-1-PBS targets FKBPL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FKBPL fusion protein Ag0112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein like
Synonyms WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2881765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UIM3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.