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Anti-Human FLT3/CD135 Rabbit Recombinant Antibody

FLT3/CD135 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241414F8

Cat no : 98106-1-RR

Print datasheet

Synonyms

FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1

验证数据展示

  • FC experiment of Reh using 98106-1-RR

    FC experiment of Reh using 98106-1-RR

    1x10^6 Reh cells (left) or U-937 cells (right) were surface stained with 0.25 ug Anti-Human FLT3 Rabbit Recombinant Antibody (98106-1-RR, Clone:241414F8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98106-1-RR

    Affinity and Kinetic Characterization of 98106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98106-1-RR against Human FLT3/CD135 were performed. The affinity constant is 1.25 nM.

经过测试的应用

Positive FC detected inReh cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98106-1-RR targets FLT3/CD135 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fms-related tyrosine kinase 3
Synonyms FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1
Calculated Molecular Weight 993 aa, 113 kDa
GenBank Accession NumberBC036028
Gene Symbol FLT3
Gene ID (NCBI) 2322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36888
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

实验方案

Product Specific Protocols
FC protocol for FLT3/CD135 antibody 98106-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols