验证数据展示
经过测试的应用
| Positive FC detected in | Reh cells |
For other applications, we recommend the unconjugated version of this antibody, 98106-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98106 targets FLT3/CD135 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | fms-related tyrosine kinase 3 |
| 别名 | FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1 |
| 计算分子量 | 993 aa, 113 kDa |
| GenBank蛋白编号 | BC036028 |
| 基因名称 | FLT3 |
| Gene ID (NCBI) | 2322 |
| RRID | AB_3673684 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P36888 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 FLT3/CD135 antibody CL647-98106 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |