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FMNL2 Monoclonal antibody

FMNL2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E8C12

Cat no : 68551-1-Ig

Print datasheet

Synonyms

FHOD2, FLJ37546, FMNL2, formin like 2, Formin like protein 2, KIAA1902

验证数据展示

  • WB analysis using 68551-1-Ig

    WB analysis using 68551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of hTERT-RPE1 using 68551-1-Ig

    WB analysis of hTERT-RPE1 using 68551-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, hTERT-RPE1 cells, HEK-293 cells, A549 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68551-1-Ig targets FMNL2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen FMNL2 fusion protein Ag34164 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name formin-like 2
Synonyms FHOD2, FLJ37546, FMNL2, formin like 2, Formin like protein 2, KIAA1902
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC167159
Gene Symbol FMNL2
Gene ID (NCBI) 114793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Formin-like 2 (FMNL2), also known as FRL3 or FHOD2, a member of the diaphanous-related formins, contains a GTPase-binding domain and autoregulatory domains, and is proposed to function as a downstream effector of Rho family guanosine triphosphatases (GTPases) (PMID:15950879). FMNL2 mRNA is expressed in many normal tissues and dysregulated in several cancers such as colorectal cancer, melanoma and involved in invasive behaviours and progression of cancer cells.

实验方案

Product Specific Protocols
WB protocol for FMNL2 antibody 68551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols