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CoraLite® Plus 488-conjugated FMO4 Monoclonal antibody

FMO4 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D12E10

Cat no : CL488-67881

Print datasheet

Synonyms

Hepatic flavin-containing monooxygenase 4, FMO2, EC:1.14.13.8, Dimethylaniline monooxygenase [N-oxide-forming] 4, 2D12E10

验证数据展示

  • IF Staining of mouse liver using CL488-67881

    IF Staining of mouse liver using CL488-67881

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 FMO4 antibody (CL488-67881, Clone: 2D12E10 ) at dilution of 1:200.

  • IF Staining of mouse liver using CL488-67881

    IF Staining of mouse liver using CL488-67881

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 FMO4 antibody (CL488-67881, Clone: 2D12E10 ) at dilution of 1:200.

  • FC experiment of HuH-7 using CL488-67881

    FC experiment of HuH-7 using CL488-67881

    1X10^6 HuH-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FMO4 (CL488-67881, Clone:2D12E10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHuH-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67881-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67881 targets FMO4 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen FMO4 fusion protein Ag7935 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name flavin containing monooxygenase 4
Synonyms Hepatic flavin-containing monooxygenase 4, FMO2, EC:1.14.13.8, Dimethylaniline monooxygenase [N-oxide-forming] 4, 2D12E10
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC002780
Gene Symbol FMO4
Gene ID (NCBI) 2329
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31512
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FMO4 antibody CL488-67881Download protocol
FC protocol for CL Plus 488 FMO4 antibody CL488-67881Download protocol
Standard Protocols
Click here to view our Standard Protocols